The Methanolic Extract of Hemidesmus Indicus and Its Fraction Induces Antihepatotoxic Activity in Vivo : Possible Involvement of Antioxidant Action

Authors

  • Jagdish R. Baheti  Kamla Nehru College of Pharmacy, Butibori Nagpur, Maharashtra, India

Keywords:

H. indicus, Hepatoprotective, DPPH assay, CCl4, Free radical scavenger

Abstract

Aim: To evaluate methanolic extract of Hemidesmus indicus and its different fractions for their hepatoprotective activity against CCl4 induced hepatotoxicity and to find out the better efficacious fraction. The study was also aimed to probe possible mechanism behind offered hepatoprotection by extract and fraction.

Method: The methanolic extract was prepared and fractionated using the solvents of varying polarity like toluene, chloroform, ethyl acetate and n-butanol and tested for hepatoprotective activity against CCl4 induced liver damage in the rats. Extent of hepatic damage was assessed by levels of SGPT, SGOT, ALP, Total Bilirubin and Direct Bilirubin and histopathologic study of liver sections. Probable mechanism was investigated by carrying out free radical scavenging activity of extract and its fractions using different invitro models like DPPH, NO and superoxide scavenging activity.

Result: There was a significant increase in the levels of serum GOT, GPT after administration of CCl4 (0.7ml/kg) to rats. Methanolic extract (100mg/kg) and (250mg/kg) and toluene fraction 50mg/kg (HM) produced significant reduction in the level of these enzymes. Methanolic extract and its toluene fraction also preserves the structural integrity of the hepatocellular membrane as revealed from histological studies. The methanolic extract and its HM (toluene fraction) exhibited significant antioxidant activity by inhibiting DPPH radical, nitric oxide and supeoxide scavenging activity

Conclusion: The methanolic extract of H. indicus roots and HM exhibited antihepatotoxic effect against CCl4 induced hepatic damage rationalise its ethnopharmacological claim and it appears that the hepatoprotection offered by H. indicus may be related to its antioxidant activity.

References

  1. Warrier PK, Nambiar VPK, Ramankutty C.Indian Medicinal Plants-Compendium of 500 species, Delhi, Orient Longman; 1994. . 3141.
  2. Anoop Austin. A review on Indian Sarsaparilla, Hemidesmus indicus (L.)R.Br. J Bio Sciences 2008; 8: 1-12.
  3. Nadkarni K M, Nadkarni A K, Chopra R N. Indian Materia Medica. 1st ed. Popular Publication, Bombay, 1954. p.619.
  4. Satyavati GV, Gupta AK Tondon N. editors In Medicinal plants of India. ICMR, New Delhi, 1987 p 13-7.
  5. Sivarajan VV, Balachandran I. Ayurvedic Drugs and their Plant Sources Oxford and IBH Publisher, Bombay 1994.
  6. Datta MK, Sen TK Sikdar S. Some preliminary observations on the anti-inflammatory properties of H. indicus in rats. Indian J of Pharmacol 1982; 14: 78-82.
  7. Alam MI, Gomes A. Viper venom-induced inflammation and inhibition of free radical formation by pure compound 2-hyroxy-4-mthoxy-benzoic acid isolated and purified from anantamul H. indicus root extract. Toxicon 1998; 36: 207-9.
  8. Prabakan M, Anandan R, Devaki T. Protective effect of H. indicus against rifampicin and isoniazid induced hepatotoxicity in rats. Fitoterapia 2000; 711: 55-9.
  9. Ravishankara MN, Shrivastava N, Padh H, Rajani M. Evaluation of antioxidant properties of root bark H. indicus anantmul. Phytomedicine 2002; 9: 153-7.
  10. Das PC, Joshi PC, Mandal S, Das A, Chatterjee A. New coumarino-lignoids from H. indicus. Indian J of Chem 1992; 31B: 342-5.
  11. Alam MI, Auddy B, Gomes A. Isolation, purification and partial characterization of Viper venom inhibiting factor from root extract of the Indian medicinal plants Sarsaparila H. indicus. Toxicon 1994; 32: 1551-6.
  12. Roy SK, Ali M, Sharma MP, Ramachandram R. New pentacyclic triterpines from the roots of H indicus. Pharmazie 2001;56: 244-6.
  13. Prakash K, Sethi A, Deepak K, Khare A, Khare MP. Two pregnane glycosides from H indicus. Phytochemistry 1991;30: 297-1.
  14. Chandra R, Deepak D, Khare A. Pregnane glycosides from H indicus. Phytochemistry. 1994;35: 1545-8.
  15. Deepak D, Shrivastava S, Khare A. Pregnane glycosides from H indicus. Phytochemistry 1997;44: 145-8.
  16. Singler P, Saksena R, Deepak D, Khare A. C-21steroidal glycosides from H indicus. Phytochemistry 2000; 54: 983-6.
  17. Ray AB, Chatopadhyay SK, Kumar S, Konno C, Kiso Y. Tetrahedron 1985;41: 222-25.
  18. Recknagel RO, Glende EA. Spectrophotometric detection of lipid conjugated dienes. Methods Enzymol 1984;105: 331-335.
  19. Smuckler EA. Alcoholic drink: Its production and effects. Fed. Proe 1975;34: 2038-2044.
  20. Doreswamy R, Sharma D. Plant drugs for liver disorders management. Indian Drugs 199532: 139-44.
  21. Handa SS, Sharma A, Chakraborti KK. Plant Drugs in liver disorders. Fitoterapia 1986;57: 307-351.
  22. Baheti JR, Goyal RK, Shah GB. Investigation of Hepatoprotective activity of Hemidesmus indicus R. Br. Indian J of Expt Biol 2006;44:
  23. Osawa T, Kavakishi S, Namiki M. Antimutagenesis and anticarcinogenesis mechanisms II 1990. P.139.
  24. Aldridge WL. Mechanism of toxicity: New concepts are required in toxicology. Trends Pharmacol Sci 1981;2: 228-36.
  25. Harborne JB. In: Phytochemical methods. London: Chapman and Hall Ltd; 1973.p.52-105.
  26. Singh K, Khanna AK, Chander R. Hepatoprotective activity of ellagic acid against carbon tetrachloride induced hepatotoxicity in rats. Indian J Expt Biol 1999;37: 1025-28.
  27. Majumdar AM, Anuradha S Upadhye, Pradhan A M. Effect of Azadirachta indica leaf extract on carbontetrachloride induced hepatic damage in rats. Indian J of Pharma Sci. 1998; 60 :( 6) 363-7.
  28. Bonina F, Saija A, Lo Cascio R, Rapisarda P, Dederen JC. In vitro antioxidant activity and in vivo photoprotective effect of a red orange extract. Intl J Cos Sci 1998;20: 331-42.
  29. Beauchamp C, Fridovich I. Superoxide dismutase : Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 1971;44: 276-277.
  30. Green LC, Wagner DA, Glogowiski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and nitrate [15N] in Biological fluids. Anal Biochem 1982 ;126:131-7.
  31. Ohkawa I, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 1979;95: 351-8.
  32. Brattin WJ, Glende EA Jr, Recknagel RO. Pathological mechanisms in cabon tetrachloride hepatotoxicity. Free Radic Biol Med 1985;1: 27-38.
  33. Packer JE, Slater TF, Wilson RL. Reaction of CCl4 related peroxy free radical with amino acids. Life Science 1978;23: 2617-28.
  34. Williams AT, Burk RF. Carbon tetrachloride hepatotoxicity: an example of free radical-medicated injury. Seminar in Liver Disease 1990;10: 279-84.
  35. Recknagel RO. A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci 1983;33(5): 401-418.
  36. Mailing HM, Eichelbaum FM, Saul W, Gipes IG, Brown GAB, Gillette JR.: Nature of protection against carbon tetrachloride induced hepatotoxicity by pretreatment with dibenamine (N-[2-chloroethyl]dibenzylamine). Biochem Pharmacol 1974;23(10): 1457-66.
  37. Brattin WJ, Glende EA Jr, Recknagel RO. Pathological mechanisms in cabon tetrachloride hepatotoxicity. Free Radic Biol Med 1985;1: 27-38.
  38. Zimmerman HJ, Seeff LB. Enzymes in hepatic disease. In: Coodley, L. editors. Diagnostic Enzymology. Lea and Febiger, Philadelphia, 1970.p. 1-38.
  39. Halliwell H. Free radicals, and human disease: curiosity, cause, or consequence? Lancet. 1994 ;344: 721-4.
  40. Cristina Nencini, Andrea Menchiari, Gian Gabriele Franchi, Lucia Micheli. In vitro antioxidant activity of aged extracts of some Italian Allium species. Plant Foods Hum Nutr 2011;66:11–16
  41. Moncada A, Palmer RMJ, Higgs EA. Nitric oxide: Physiology, Pathophysiology and Pharmacology. Pharmacological reviews 1991;43: 109-42.
  42. Sessa WC, Harrison JK, Luthin DR, Pollack JS, Lynch KR. Genomic analysis and expression patterns reveal distinct genes for endothelial and brain nitric oxide synthase. Hypertension 1993 ;21: 934-8.
  43. Larson RA. Antioxidants from higher plants. Phytochemistry. 1988 ;27(4): 969-78.

International Journal of Scientific Research in Science and Technology

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Published

2018-03-30

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Volume 4, Issue 7, March-April-2018

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Research Articles

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[1]
Jagdish R. Baheti, " The Methanolic Extract of Hemidesmus Indicus and Its Fraction Induces Antihepatotoxic Activity in Vivo : Possible Involvement of Antioxidant Action, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 7, pp.940-948, March-April-2018.